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# BBO-Bioinformatics-Bash-Oneliner
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Hi bioinformaticans and bash learner, welcome to BBO, Bioinformatics Bash Oneliner learning station. I started studying bioinformatics data three years ago, and I was amazed by those single-word bash commands which are much faster than my dull scripts, so i started and insist using bash. Not all the code here is oneliner (if the ';' counts..), but i put effort on making them brief and fast.
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This blog will focus on bash commands for parsing biological data, most of which are tsv files (tab-separated values); some of them are for Ubuntu system maintaining. I have been recording the bash commands on my notebook, but putting them on web will help others and myself to 'Ctrl +F '. I apologize that there won't be any citation for the codes, coz i haven't make any record of it, but they are probably from dear Google and Stackoverflow.
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English and bash are not my first language, so... correct me anytime, thank you
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In case you would like to check up and like my stupid questions on Stackoverflow, here's my page:
http://stackoverflow.com/users/4290753/once
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And if you want to check out stylish BBO:
http://bbonut.blogspot.tw/#system
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##Handy Bash oneliner commands for tsv file editing
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- [Grep ](#grep )
- [Sed ](#sed )
- [Awk ](#awk )
- [Xargs ](#xargs )
- [Find ](#find )
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- [Loops ](#loops )
- [Download ](#download )
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- [Others ](#others )
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- [System ](#system )
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##Grep
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#####extract text bewteen words (e.g. w1,w2)
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grep -o -P '(?< =w1).*(?=w2)'
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#####grep lines without word (e.g. bbo)
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grep -v bbo
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#####grep and count (e.g. bbo)
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grep -c bbo filename
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#####insensitive grep (e.g. bbo/BBO/Bbo)
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grep -i "bbo" filename
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#####count occurrence (e.g. three times a line count three times)
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grep -o bbo filename
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#####COLOR the match (e.g. bbo)!
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grep --color bbo filename
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#####grep search all files in a directory(e.g. bbo)
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grep -R bbo /path/to/directory
or
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grep -r bbo /path/to/directory
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#####search all files in directory, only output file names with matches(e.g. bbo)
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grep -Rh bbo /path/to/directory or
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grep -rh bbo /path/to/directory
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#####grep OR (e.g. A or B or C or D)
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grep 'A\|B\|C\|D'
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#####grep AND (e.g. A and B)
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grep 'A.*B'
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#####grep all content of a fileA from fileB
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grep -f fileA fileB
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#####grep a tab
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grep $'\t'
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##Sed
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[[back to top ](#handy-bash-oneliner-commands-for-tsv-file-editing )]
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#####remove lines with word (e.g. bbo)
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sed "/bbo/d" filename
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#####edit infile (edit and save)
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sed -i "/bbo/d" filename
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#####when using variable (e.g. $i), use double quotes " "
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e.g. add >$i to the first line (to make a FASTA file)
sed "1i >$i"
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//notice the double quotes! in other examples, you can use a single quote, but here, no way!
//'1i' means insert to first line
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#####delete empty lines
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sed '/^\s*$/d'
or
sed 's/^$/d'
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#####delete last line
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sed '$d'
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#####add \n every nth character (e.g. every 4th character)
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sed 's/.\{4\}/& \n/g'
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#####substitution (e.g. replace A by B)
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sed 's/A/B/g' filename
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#####select lines start with string (e.g. bbo)
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sed -n '/^@S/p'
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#####delete lines with string (e.g. bbo)
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sed '/bbo/d' filename
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#####print every nth lines
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sed -n '0~3p' filename
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//catch 0: start; 3: step
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#####print every odd # lines
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sed -n '1~2p'
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#####print every third line including the first line
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sed -n '1p;0~3p'
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#####remove leading whitespace and tabs
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sed -e 's/^[ \t]*//'
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//notice a whitespace before '\t'!!
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#####remove only leading whitespace
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sed 's/ *//'
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//notice a whitespace before '*'!!
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#####remove ending commas
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sed 's/,$//g'
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#####add a column to the end
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sed "s/$/\t$i/"
//$i is the valuable you want to add
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e.g. add the filename to every last column of the file
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for i in $(ls);do sed -i "s/$/\t$i/" $i;done
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#####remove newline\ nextline
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sed ':a;N;$!ba;s/\n//g'
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#Awk
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[[back to top ](#handy-bash-oneliner-commands-for-tsv-file-editing )]
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#####set tab as field separator
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awk -F $'\t'
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#####output as tab separated (also as field separator)
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awk -v OFS='\t'
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#####pass variable
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a=bbo;b=obb;
awk -v a="$a" -v b="$b" "$1==a & & $10=b' filename
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#####print number of characters on each line
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awk '{print length ($0);}' filename
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#####find number of columns
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awk '{print NF}'
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#####reverse column order
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awk '{print $2, $1}'
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#####check if there is a comma in a column (e.g. column $1)
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awk '$1~/,/ {print}'
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#####split and do for loop
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awk '{split($2, a,",");for (i in a) print $1"\t"a[i]} filename
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#####print all lines before nth occurence of a string (e.g stop print lines when bbo appears 7 times)
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awk -v N=7 '{print}/bbo/& & --N< =0 {exit}'
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#####add string to the beginning of a column (e.g add "chr" to column $3)
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awk 'BEGIN{OFS="\t"}$3="chr"$3'
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#####remove lines with string (e.g. bbo)
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awk '!/bbo/' file
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#####column subtraction
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cat file| awk -F '\t' 'BEGIN {SUM=0}{SUM+=$3-$2}END{print SUM}'
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#####usage and meaning of NR and FNR
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e.g.
fileA:
a
b
c
fileB:
d
e
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awk 'print FILENAME, NR,FNR,$0}' fileA fileB
fileA 1 1 a
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fileA 2 2 b
fileA 3 3 c
fileB 4 1 d
fileB 5 2 e
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#####and gate
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e.g.
fileA:
1 0
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2 1
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3 1
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4 0
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fileB:
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1 0
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2 1
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3 0
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4 1
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awk -v OFS='\t' 'NR=FNR{a[$1]=$2;next} NF {print $1,((a[$1]=$2)? $2:"0")}' fileA fileB
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1 0
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2 1
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3 0
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4 0
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#####round all numbers of file (e.g. 2 significant figure)
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awk '{while (match($0, /[0-9]+\[0-9]+/)){
\printf "%s%.2f", substr($0,0,RSTART-1),substr($0,RSTART,RLENGTH)
\$0=substr($0, RSTART+RLENGTH)
\}
\print
\}'
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#####give number/index to every row
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awk '{printf("%s\t%s\n",NR,$0)}'
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##Xargs
[[back to top ](#handy-bash-oneliner-commands-for-tsv-file-editing )]
set tab as delimiter (default:space)
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xargs -d\t
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#####display 3 items per line
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echo 1 2 3 4 5 6| xargs -n 3
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//1 2 3
4 5 6
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#####prompt before execution
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echo a b c |xargs -p -n 3
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#####print command along with output
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xargs -t abcd
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///bin/echo abcd
//abcd
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#####with find and rm
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find . -name "*.html"|xargs rm -rf
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delete fiels with whitespace in filename (e.g. "hello 2001")
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find . -name "*.c" -print0|xargs -0 rm -rf
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#####show limits
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xargs --show-limits
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#####move files to folder
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find . -name "*.bak" -print 0|xargs -0 -I {} mv {} ~/old
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or
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find . -name "*.bak" -print 0|xargs -0 -I file mv file ~/old
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#####move first 100th files to a directory (e.g. d1)
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ls |head -100|xargs -I {} mv {} d1
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#####parallel
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time echo {1..5} |xargs -n 1 -P 5 sleepa lot faster than
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time echo {1..5} |xargs -n1 sleep
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#####copy all files from A to B
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find /dir/to/A -type f -name "*.py" -print 0| xargs -0 -r -I file cp -v -p file --target-directory=/path/to/B
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//v: verbose|
//p: keep detail (e.g. owner)
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#####with sed
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ls |xargs -n1 -I file sed -i '/^Pos/d' filename
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#####add the file name to the first line of file
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ls |sed 's/.txt//g'|xargs -n1 -I file sed -i -e '1 i\>file\' file.txt
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#####count all files
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ls |xargs -n1 wc -l
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#####to filter txt to a single line
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ls -l| xargs
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#####count files within directories
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echo mso{1..8}|xargs -n1 bash -c 'echo -n "$1:"; ls -la "$1"| grep -w 74 |wc -l' --
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// "--" signals the end of options and display further option processing
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#####download dependencies files and install (e.g. requirements.txt)
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cat requirements.txt| xargs -n1 sudo pip install
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#####count lines in all file, also count total lines
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ls|xargs wc -l
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##Find
[[back to top ](#handy-bash-oneliner-commands-for-tsv-file-editing )]
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#####list all sub directory/file in the current directory
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find .
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#####list all files under the current directory
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find . -type f
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#####list all directories under the current directory
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find . -type d
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#####edit all files under current directory (e.g. replace 'www' with 'ww')
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find . name '*.php' -exec sed -i 's/www/w/g' {} \;
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if no subdirectory
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replace "www" "w" -- *
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//a space before *
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#####find and output only filename (e.g. "mso")
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find mso*/ -name M* -printf "%f\n"
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#####find and delete file with size less than (e.g. 74 byte)
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find . -name "*.mso" -size -74c -delete
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//M for MB, etc
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##Loops
[[back to top ](#handy-bash-oneliner-commands-for-tsv-file-editing )]
#####while loop, column subtraction of a file (e.g. a 3 columns file)
while read a b c; do echo $(($c-$b));done < < (head filename)
//there is a space between the two '< 's
#####while loop, sum up column subtraction
i=0; while read a b c; do ((i+=$c-$b)); echo $i; done < < (head filename)
#####if loop
if (($j==$u+2))
//(( )) use for arithmetic operation
if [[$age >21]]
//[[ ]] use for comparison
#####for loop
for i in $(ls); do echo file $i;done
##Download
[[back to top ](#handy-bash-oneliner-commands-for-tsv-file-editing )]
#####download all from a page
wget -r -l1 -H -t1 -nd -N -np -A mp3 -e robots=off http://example.com
//-r: recursive and download all links on page
//-l1: only one level link
//-H: span host, visit other hosts
//-t1: numbers of retries
//-nd: don't make new directories, download to here
//-N: turn on timestamp
//-nd: no parent
//-A: type (seperate by ,)
//-e robots=off: ignore the robots.txt file which stop wget from crashing the site, sorry example.com
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##Others
[[back to top ](#handy-bash-oneliner-commands-for-tsv-file-editing )]
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#####remove newline / nextline
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tr --delete '\n' < input.txt > output.txt
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#####replace newline
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tr '\n' ' ' < filename
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#####compare files (e.g. fileA, fileB)
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diff fileA fileB
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//a: added; d:delete; c:changed
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or
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sdiff fileA fileB
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//side-to-side merge of file differences
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#####number a file (e.g. fileA)
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nl fileA
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or
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nl -nrz fileA
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//add leading zeros
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#####combine/ paste two files (e.g. fileA, fileB)
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paste fileA fileB
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//default tab seperated
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#####reverse string
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echo 12345| rev
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#####read .gz file without extracting
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zmore filename
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or
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zless filename
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#####run in background, output error file
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(command here) 2>log &
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or
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(command here) 2>& 1| tee logfile
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or
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(command here) 2>& 1 >>outfile
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//0: standard input; 1: standard output; 2: standard error
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#####send mail
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echo 'heres the content'| mail -A 'file.txt' -s 'mail.subject' me@gmail.com
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//use -a flag to set send from (-a "From: some@mail.tld")
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.xls to csv
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xls2csv filename
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#####append to file (e.g. hihi)
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echo 'hihi' >>filename
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#####make BEEP sound
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speaker-test -t sine -f 1000 -l1
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set beep duration
(speaker-test -t sine -f 1000) & pid=$!;sleep 0.1s;kill -9 $pid
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#####history edit/ delete
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~/.bash_history
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or
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history -d [line_number]
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#####get last history/record filename
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head !$
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#####clean screen
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clear
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or
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Ctrl+l
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#####send data to last edited file
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cat /directory/to/file
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echo 100>!$
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#####run history number (e.g. 53)
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!53
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#####run last command
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!!
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#####run last command that began with (e.g. cat filename)
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!cat
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or
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!c
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//run cat filename again
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#####extract .xf
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1.unxz filename.tar.xz
2.tar -xf filename.tar
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#####install python package
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pip install packagename
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#####random order (lucky draw)
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for i in a b c d e; do echo $i; done| shuf
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#####echo a random number
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echo $RANDOM
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#####Download file if necessary
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data=file.txt
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url=http://www.example.com/$data
if [! -s $data];then
echo "downloading test data..."
wget $url
fi
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#####wget to a filename (when a long name)
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wget -O filename "http://example.com"
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#####wget files to a folder
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wget -P /path/to/directory "http://example.com"
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#####delete current bash command
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Ctrl+U
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or
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Ctrl+C
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or
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Alt+Shift+#
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//to make it to history
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#####add things to history (e.g. "addmetohistory")
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#addmetodistory
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//just add a "#" before~~
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#####sleep awhile or wait for a moment or schedule a job
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sleep 5;echo hi
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#####count the time for executing a command
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time echo hi
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#####backup with rsync
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rsync -av filename filename.bak
rsync -av directory directory.bak
rsync -av --ignore_existing directory/ directory.bak
rsync -av --update directory directory.bak
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//skip files that are newer on receiver (i prefer this one!)
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#####make all directories at one time!
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mkdir -p project/{lib/ext,bin,src,doc/{html,info,pdf},demo/stat}
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//-p: make parent directory
//this will create project/doc/html/; project/doc/info; project/lib/ext ,etc
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#####run command only if another command returns zero exit status (well done)
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cd tmp/ & & tar xvf ~/a.tar
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#####run command only if another command returns non-zero exit status (not finish)
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cd tmp/a/b/c ||mkdir -p tmp/a/b/c
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#####extract to a path
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tar xvf -C /path/to/directory filename.gz
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#####use backslash "\" to break long command
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cd tmp/a/b/c \
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> || \
>mkdir -p tmp/a/b/c
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#####get pwd
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VAR=$PWD; cd ~; tar xvf -C $VAR file.tar
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//PWD need to be capital letter
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#####list file type of file (e.g. /tmp/)
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file /tmp/
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//tmp/: directory
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#####bash script
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#!/bin/bash
file=${1#*.}
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//remove string before a "."
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file=${1%.*}
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//remove string after a "."
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#####search from history
Ctrl+r
#####python simple HTTP Server
python -m SimpleHTTPServer
#####variables
{i/a/,}
e.g. replace all
{i//a/,}
//for variable i, replace all 'a' with a comma
#####read user input
read input
echo $input
#####generate sequence 1-10
seq 10
#####sum up input list (e.g. seq 10)
seq 10|paste -sd+|bc
#####find average of input list/file
i=`wc -l filename|cut -d ' ' -f1`; cat filename| echo "scale=2;(`paste -sd+`)/"$i|bc
#####generate all combination (e.g. 1,2)
echo {1,2}{1,2}
//1 1, 1 2, 2 1, 2 2
#####generate all combination (e.g. A,T,C,G)
set = {A,T,C,G}
group= 5
for ((i=0; i< $group; i++));do
repetition=$set$repetition;done
bash -c "echo "$repetition""
#####read file content to variable
foo=$(< test1 )
#####echo size of variable
echo ${#foo}
#####array
declare -A array=()
#####send a directory
scp -r directoryname user@ip:/path/to/send
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##System
[[back to top ](#handy-bash-oneliner-commands-for-tsv-file-editing )]
#####snapshot of the current processes
ps
#####check graphics card
lspci
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#####show IP address
$ip add show
or
ifconfig
#####check system version
cat /etc/*-release
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#####Linux Programmer's Manuel: hier- description of the filesystem hierarchy
man hier
#####list job
jobs -l
#####export PATH
export PATH=$PATH:~/path/you/want
#####make file execuable
chmod +x filename
//you can now ./filename to execute it
#####list screen
screen -d -r
#####echo screen name
screen -ls
#####check system (x86-64)
uname -i
#####surf the net
links www.google.com
#####add user, set passwd
useradd username
passwd username
#####edit variable for bash, (e.g. displaying the whole path)
1. joe ~/.bash_profile
2. export PS1='\u@\h:\w\$'
//$PS1 is a variable that defines the makeup and style of the command prompt
3. source ~/.bash_profile
#####edit environment setting (e.g. alias)
1. joe ~/.bash_profile
2. alias pd="pwd" //no more need to type that 'w'!
3. source ~/.bash_profile
#####list environment variables (e.g. PATH)
$echo $PATH
//list of directories separated by a colon
#####list all environment variables for current user
$env
#####show partition format
lsblk
#####soft link program to bin
ln -s /path/to/program /home/usr/bin
//must be the whole path to the program
#####show hexadecimal view of data
hexdump -C filename.class
#####jump to different node
rsh node_name
#####check port (active internet connection)
netstat -tulpn
#####find whick link to a file
readlink filename
#####check where a command link to (e.g. python)
which python
#####list total size of a directory
du -hs .
or
du -sb
#####copy directory with permission setting
cp -rp /path/to/directory
#####store current directory
pushd . $popd ;dirs -l
#####show disk usage
df -h
or
du -h
or
du -sk /var/log/* |sort -rn |head -10
#####show current runlevel
runlevel
#####switch runlevel
init 3 or
telinit 3
#####permanently modify runlevel
1. edit /etc/init/rc-sysinit.conf
2. env DEFAULT_RUNLEVEL=2
#####become root
su
#####become somebody
su somebody
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=-=-=-=-=-A lot more coming!! =-=-=-=-=-=-=-=-=-=waitwait-=-=-=-=-=-=-=-=-=-